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Indoor air measurement for cultivable mould fungi in a day care centre

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Analysis in accordance with VDI 4300 Sheet 10 by M&P Umwelttechnik Heidelberg

After leaks were detected in the roof area of a daycare centre, visible moisture damage appeared on the ceilings in several rooms. In addition to visual abnormalities such as discolouration and areas of moisture penetration, employees reported unpleasant odours, which were particularly noticeable when entering the building in the morning.

Against this background, the M&P Umwelttechnik GmbH – Part of SIERA Alliancewith a technical assessment and the performance of a microbiological indoor air analysis.

Particularly in sensitive facilities such as childcare centres, an objective, comprehensible and standard-oriented assessment is crucial. In the case of moisture damage, the focus is not only on the building fabric, but also on the question of whether the indoor air could be contaminated by microbiological influences.

Initial situation: moisture damage and noticeable odours

During the inspection, damage was identified that indicated a probable ingress of moisture as a result of the roof leak. In several rooms Visible water damage to the ceilings recognisable. In addition to this Odour abnormalities described, which were particularly noticeable in the morning when entering the rooms.

In practice, such constellations are a typical reason to initiate a microbiological indoor air analysis – not to jump to conclusions, but to enable a professionally sound clarification based on a recognised procedure.

The realisation was carried out by the M&P Umwelttechnik GmbH – Part of SIERA Alliance as part of a standardised procedure in accordance with VDI 4300 Sheet 10.

Scope of investigation: nine affected rooms

During the site inspection, a total of nine rooms identified in which:

  • there was visible water damage to the ceilings,
  • moisture ingress as a result of the roof leak was likely,
  • and odour abnormalities were described.

To assess possible microbial contamination of the indoor air, M&P Umwelttechnik Heidelberg – a member of the SIERA Alliance – carried out the following tests in these nine affected rooms Indoor air measurements for cultivable moulds in accordance with VDI 4300 Sheet 10 through.

Methodological procedure: standardised sampling according to VDI 4300 Sheet 10

Sampling was standardised using Impaction process. A defined volume of air is applied to suitable nutrient media in order to produce viable mould spores. colony-forming units (CFU/m³) – to record.

In order to be able to meaningfully categorise the indoor values, a supplementary Outside air sample as reference is used. This allows indoor concentrations to be assessed in relation to natural background levels.

The investigation comprised the following steps:

  1. Definition of representative measuring points in each of the nine rooms
  2. Documentation of the indoor climate conditions
  3. Laboratory incubation and differentiation of cultivable moulds
  4. Comparison of indoor and outdoor air

Table: Investigation steps and target reference

Examination step (VDI 4300 Sheet 10)Purpose of the „Indoor air measurement daycare centre“ project“
Define representative measuring points per roomEnable comparability of samples from nine affected rooms
Document indoor climate conditionsClearly record the framework conditions of the measurement
Impaction process with defined air volumeDetection of cultivable moulds (CFU/m³)
Laboratory incubation and differentiationDetermination/differentiation of cultivable moulds
Outside air sample as referenceClassification of indoor concentrations to background levels
Comparison of indoor and outdoor airCheck whether there are indications of typical indoor sources

(The table only summarises the process described in the source text, without claiming additional results).

Aim of the investigation: precautionary health protection

The indoor air measurement served to clarify professionally whether, as a result of the moisture damage, a Relevant microbiological contamination of the indoor air is available.

In a day care centre in particular, the precautionary health protection of children and employees. The focus was therefore on the question of whether indications of Typical interior mould source exist and whether, if applicable Further structural or hygiene measures are required.

The processing was carried out by the M&P Umwelttechnik GmbH – Part of SIERA Alliance with the aim of creating a structured, comprehensible basis for decision-making.

Why a standardised approach is crucial

The application of the VDI 4300 Sheet 10 guarantees a structured, comprehensible and recognised procedure for assessing possible mould contamination in indoor spaces.

For operators and managers of a childcare centre, this approach means in particular

  • TransparencyProcedure and evaluation steps are clearly documented.
  • ComparabilityIndoor values can be considered in relation to the outside air.
  • TraceabilityStandardised measurement and evaluation steps support a reliable professional assessment.

The study therefore does not provide any „assumptions“, but rather a methodologically sound basis for deriving sensible next steps – should these become necessary.

By carrying out indoor air measurements in nine rooms affected by moisture damage, M&P Umwelttechnik Heidelberg – a member of the SIERA Alliance – provided a standardised and technically sound assessment of indoor air quality.

The application of the VDI 4300 Sheet 10 guarantees a structured, comprehensible and recognised procedure for assessing possible mould contamination in indoor spaces.

If moisture damage, abnormal odours or suspected microbiological influences occur in your property, the M&P Umwelttechnik GmbH – Part of SIERA Alliance with standardised indoor air measurements and technical assessment.

Get in touch with us for an initial categorisation and the planning of a standard-oriented investigation.

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A Message from the Founder: Florian von Tucher

In the mid-2000s my involvement in development aid took me to some of the most remote and impoverished regions of the world. 

Northern Tibet, Mongolia, and Western China – where I was involved with the implementation of decentralised wastewater treatment systems, I realised I needed a deeper purpose. Though I later found success in real estate development, the desire to make a lasting impact never left me.  

A pivotal moment occurred when I was invited to Ghana by my friend and mentor, Cardinal Peter Turkson, who was the head of the Dicastery for the Promotion of Integral Human Development of the Catholic Church at the time. He has since been appointed the Pontifical Chancellor of the Academy of Sciences and the Pontifical Chancellor of Social Sciences.  

Cardinal Turkson had a profound influence on me. His invitation gave me the opportunity to witness firsthand the development needs of the country. We reflected on my experiences in China, and together, we envisioned a model of development that would take root in one community and gradually expand. We believed that small, strategic steps could lead to lasting transformation – just like the biblical parable of the mustard seed, which grows into something far greater than its humble beginnings. 

Cardinal Turkson’s steadfast belief in this vision and encouragement became the base upon which the Mustard Seed Foundation was built. His unwavering support, wisdom, and guidance helped shape not just the mission of the Foundation, but my personal journey as well. 

With the encouragement of the Cardinal and the Integral Human Development (IHD) office, we initially operated with the IHD before establishing the Mustard Seed Foundation as a stand-alone organisation in Germany. We have been fortunate to receive support from numerous European donors, a humble reminder that our mission is not just about individual efforts – it is about collective impact. 

Collaboration has been a cornerstone of our work. We have partnered with organisations like Caritas and Rotary International to extend our reach. One of our most impactful collaborations has been with M&P Group, who donate their engineering concepts, project supervision, and high-quality technical execution, allowing 100% of donor contributions to go into the projects themselves. 

One such initiative is the Clean Water Initiative, launched in partnership with M&P Group. In 2024, we completed a well in Ndoss, Senegal, significantly improving agricultural efficiency and empowering the local community. This project epitomises our commitment to sustainable solutions – starting with clean water and gradually building infrastructure that supports long-term development. 

Our work aligns closely with the United Nations Sustainable Development Goals (SDGs), focusing on poverty alleviation, economic empowerment, environmental sustainability, and humanitarian aid. Our model is simple but effective: start with one project and expand, year by year, to create an ecosystem of support. A water well leads to a school, which leads to renewable energy solutions, which, in turn, fosters economic opportunities. Over time, these efforts cumulatively transform entire regions. 

The Mustard Seed Foundation is a testament to what can be achieved with nothing more than a vision, a strong commitment, and the faith of a mustard seed. Yet, none of this would have been possible without the belief and encouragement of Cardinal Peter Turkson. His unwavering faith in our mission gave me the courage to persevere through challenges and continue expanding our impact. As we continue our work, we remain driven by the belief that small beginnings can yield great outcomes, inspiring hope and lasting change in the communities we serve. His legacy of faith, vision, and commitment to human dignity is deeply woven into every initiative we undertake.